Volume 11 Supplement 4

UT-ORNL-KBRIN Bioinformatics Summit 2010

Open Access

Statistical analysis of multiple significance test methods for differential proteomics

  • Bing Wang1,
  • Fahim Mohammad2,
  • Jun Zhang1,
  • Xinmin Yin1,
  • Eric Rouchka2 and
  • Xiang Zhang1
BMC Bioinformatics201011(Suppl 4):P30

DOI: 10.1186/1471-2105-11-S4-P30

Published: 23 July 2010

Background

In current proteomics research, a big challenge is to differentiate the correlative proteins for a given biological function from all existing proteins, and if it does, how strong is the relationship between the proteins and function. Statistical significance testing can be used to address this question. However, every traditional statistical test method may suffer from the inability to identify important differentially expressed proteins if the biological samples do not completely meet the assumptions of each test method [1]. To detect the regulated proteins for differential proteomics, we analyze multiple significance test methods and discover some significance proteins.

Results

We use the four statistical methods, i.e., Kolmogorov-Smirnov test (KS-test), Baumgartner-Weib-Schindler test (BWS-test), T-test, Brunner-Munzel test (BM-test) to measure the difference of the expression level of individual protein under two experimental conditions, respectively. The results had been successfully used for the discovery of protein biomarkers in breast cancer.

Acknowledgements

This work was supported by a grant from National Institute of Health (NIH) under grant number RO1GM087735 and a Competitive Enhancement Grant funded by University of Louisville.

Authors’ Affiliations

(1)
Department of Chemistry, University of Louisville
(2)
Department of Computer Engineering and Computer Science, University of Louisville

References

  1. Li J, Tang X, Zhao W, Huang J: A new framework for identifying differentially expressed genes. Pattern Recognition 2007, 40: 3249–3262. 10.1016/j.patcog.2007.01.032View ArticleGoogle Scholar

Copyright

© Zhang et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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