DCD – a novel plant specific domain in proteins involved in development and programmed cell death
© Tenhaken et al; licensee BioMed Central Ltd. 2005
Received: 04 April 2005
Accepted: 11 July 2005
Published: 11 July 2005
Recognition of microbial pathogens by plants triggers the hypersensitive reaction, a common form of programmed cell death in plants. These dying cells generate signals that activate the plant immune system and alarm the neighboring cells as well as the whole plant to activate defense responses to limit the spread of the pathogen. The molecular mechanisms behind the hypersensitive reaction are largely unknown except for the recognition process of pathogens. We delineate the NRP-gene in soybean, which is specifically induced during this programmed cell death and contains a novel protein domain, which is commonly found in different plant proteins.
The sequence analysis of the protein, encoded by the NRP-gene from soybean, led to the identification of a novel domain, which we named DCD, because it is found in plant proteins involved in d evelopment and c ell d eath. The domain is shared by several proteins in the Arabidopsis and the rice genomes, which otherwise show a different protein architecture. Biological studies indicate a role of these proteins in phytohormone response, embryo development and programmed cell by pathogens or ozone.
It is tempting to speculate, that the DCD domain mediates signaling in plant development and programmed cell death and could thus be used to identify interacting proteins to gain further molecular insights into these processes.
Plants can recognize microbial pathogens by a specific interaction system, which was historically named the gene-for-gene interaction, because particular matching genes must be present in the pathogen as well as in the plant. A successful recognition triggers a hypersensitive reaction of individual plant cells, which is a form of programmed cell death in plants. Though a dead cell on its own might already stop the growth of biotrophic pathogens more importantly the cell death program by itself generates unknown signals for neighboring cells. Thereby the plant immune system is activated locally in some cell layer around the original infection to prepare the plant cells for the next microbial attack. Often this signal from the first infection spreads throughout the whole plant and turns on a long lasting broad pathogen resistance called the systemic acquired resistance. Despite the enormous efforts to dissect the machinery for the hypersensitive reaction many details are still unknown except for the early recognition of the microbial molecules.
Often the programmed cell death in plants requires the signaling compound salicylic acid downstream of the recognition process to proceed beyond restrictions points in the cell death program . A conclusive role for salicylic acid has not been worked out but it is likely to function in signal amplification [2, 3] and transcriptional activation of genes are very likely [4, 5].
We have isolated a gene from soybean which is strongly induced during the hypersensitive reaction and serves as a marker for programmed cell death in this system . The gene is not directly responsive to salicylic acid but transcription can be amplified in the presence of this signal molecule. The gene encodes a protein consisting of two domains. The N-terminal domain is extremely rich in the amino acid asparagine (~25%) and was therefore called N-rich protein (NRP) . The exact biological function of the NRP-gene remains to be elucidated.
Here we describe the analysis of a protein domain found in the soybean NRP-protein and other plant proteins associated with development. The biological processes associated with these proteins lead us to name this novel domain DCD for their role in d evelopment and c ell d eath.
Results and discussion
Whereas the majority of DCD domains (families II, III. IV) contain a second conserved cysteine, directly following the N-terminal one, family I possess a putative functional substitute in the "central loop" of the domain (Fig. 1, green asterisks on the top of the alignment).
We could only identify the DCD domain in a variety of plants, using PSI-BLAST. The domain seems to be present in ESTs from dicots (e.g. Arabidopsis), monocots (e.g. rice), gymnosperm trees (e.g. pine), ferns, and mosses (e.g. Physcomitrella). The available sequences from algae are very limited, but the recently sequenced diatom Thalassiosira pseudonana  contains a distant member of this domain in a hypothetical protein (Fig. 2). At least the DCD domain is present from early in plant evolution before the separation of diatoms and green algae, leading to higher plants, occurred about 1 billion years ago.
For three of the proteins with a DCD domain, all clustering into group I, some biological data have been published. These proteins include the B2-protein from carrot, which was found to be strongly and early induced during the developmental shift from undifferentiated cell cultures to somatic embryogenesis . Though the exact function of the protein still has to be elucidated a role in developmental processes is supported by the finding from Arabidopsis transcript profiling with microarrays. Here the DCD containing protein At2g32910 is only weakly expressed throughout the whole life cycle of Arabidopsis except during embryogenic development. A similar pattern is observed for the gene At5g01660, which has several KELCH repeats next to the DCD domain. This gene is most abundantly expressed in embryos but also in the meristem of the shoot apex.
A second protein with a DCD domain was identified by  in pea. Here the so called Gda1 gene is strongly expressed in peas during the vegetative phase but rapidly disappears after shifting the plants into the reproductive phase. The transition is mediated by a change of the light period from short to long days. Interestingly the GDA1 gene can be rapidly induced by the phytohormone gibberellic acid, a key player in the developmental change from the vegetative to the reproductive phase in plants. The GDA1 transcript accumulated only 15 min after application of gibberellic acid, indicating that the GDA1 gene is a primary response gene to this phytohormone.
A third protein with a DCD domain was isolated by . This protein was named N-rich protein (NRP) because of the extreme high content of asparagine (~25%) in the N-terminal half in front of the DCD domain. The NRP-gene is rapidly induced during programmed cell death in soybean, caused by inoculation with avirulent bacteria. Isogenic bacteria, lacking a single Avr-gene are not recognized by soybean cells and neither trigger programmed cell death nor the induction of the NRP gene. The gene is induced early in the cell death program well before the cells lose control of their membrane integrity. Using Phytophthora as a fungal pathogen to inoculate soybean plants, the same response was found as with bacteria, indicating that the NRP-gene is responding to the cell death program rather than to specific molecules from a particular pathogen. The putative Arabidopsis ortholog (At5g42050) is induced by several stress conditions including ozone, osmotic and cold stress as indicated by publicly available transcript profiling data (Genevestigator: https://www.genevestigator.ethz.ch/). Ozone treatment leads to small lesions with cell death similar to a hypersensitive reaction caused by avirulent pathogens. A similar set of genes is activated by both inducers of programmed cell death.
The DCD domain is quite well conserved on the amino acid level throughout the plant kingdom. The domain is present in proteins with different architectures. Some of these proteins contain additional recognizable motifs, like the KELCH repeats or the ParB domain. The latter domain has been attributed to the partitioning of plasmids and chromosomes in bacteria and has a nuclease activity .
KELCH motifs are typically composed of ~50 amino acid long stretches which form a beta sheet . They occur as 5 to 7 repeats that form a beta propeller tertiary structure. KELCH motifs are widespread and have been identified in viruses, plants, fungi and mammals. Most of the characterized KELCH motifs are interfaces for protein protein interaction, often by interaction with proteins from the cytoskeleton .
The occurrence of the conserved DCD domain in plant proteins of variable length and different architecture, but present throughout the plant kingdom, suggests a role in protein-protein interaction. Transcription profiling reveals that the genes encoding a DCD domain are upregulated during plant development and programmed cell death. It is tempting to speculate, that the DCD domain mediates the signaling in these processes and could thus be used to identify interacting proteins to gain further molecular insights into these processes.
Using the protein sequence of Glycine max (gi|57898928) as query for a PSI-Blast search  after one iteration we retrieve homologs in several plant families with high significance (E-value > = 8*e-30). A conserved region of ~130 amino acids could be identified and the borders of the shared region were defined according to the PSI-BLAST pairwise alignments. Further PSI-BLAST searches with this region converge within the first iteration. A multiple sequence alignment was build using T-coffee and refined manually; additional HMM searches  with profiles based on this alignment of non redundant representatives support the findings.
Phylogenetically distant sequences of diatom (Thalassiosira pseudonana), fern (Ceratopteris richardii) and moss (Physcomitrella patens) derive from searches against genome database and EST database, respectively.
A phylogenetic tree was reconstructed using the non redundant alignment of 29 sequences (including fragments and one translation (O49932) that likely contains a frameshift at the C-terminus) in MEGA , calculated with the neighbor-joining algorithm. Similar topologies were obtained using other methods e.g minimum evolution (data not shown) and bootstrap values were calculated to test significance. The domain architecture is predicted and displayed by the Simple Modular Architecture Research Tool http://smart.embl-heidelberg.de[19, 20].
- Alvarez ME: Salicylic acid in the machinery of hypersensitive cell death and disease resistance. Plant Mol Biol 2000, 44: 429–42. 10.1023/A:1026561029533View ArticlePubMedGoogle Scholar
- Shirasu K, Nakajima H, Krishnamachari Rajasekhar V, Dixon RA, Lamb C: Salicylic acid potentiates an agonistdependent gain control that amplifies pathogen signals in the activation of defense mechanisms. Plant Cell 1997, 9: 261–270. 10.1105/tpc.9.2.261PubMed CentralView ArticlePubMedGoogle Scholar
- Tenhaken R, Rübel C: Salicylic acid is needed in hypersensitive cell death in soybean but does not act as a catalase inhibitor. Plant Physiol 1997, 115: 291–298.PubMed CentralPubMedGoogle Scholar
- Maleck K, Levine A, Eulgem T, Morgan A, Schmid J, Lawton KA, Dangl JL, Dietrich RA: The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 2000, 26: 403–410. 10.1038/82521View ArticlePubMedGoogle Scholar
- Tenhaken R, Anstätt C, Ludwig A, Seehaus K: WY-14,643 and other agonists of the peroxisome proliferator-activated receptor reveal a new mode of action for salicylic acid in soybean disease resistance. Planta 2001, 212: 888–895. 10.1007/s004250000463View ArticlePubMedGoogle Scholar
- Ludwig AA, Tenhaken R: A new cell wall located N-rich protein is strongly induced during the hypersensitive response in Glycine max L. Eur J Plant Pathol 2001, 107: 323–336. 10.1023/A:1011202225323View ArticleGoogle Scholar
- Ginalski K, Elofsson A, Fischer D, Rychlewski L: 3D-Jury: a simple approach to improve protein structure predictions. Bioinformatics 2003, 19: 1015–1018. 10.1093/bioinformatics/btg124View ArticlePubMedGoogle Scholar
- Armbrust EV, Berges JA, Bowler C, Green BR, Martinez D, et al.: The Genome of the diatom Thalassiosira pseudonana : Ecology, Evolution, and Metabolism. Science 2004, 306: 79–86. 10.1126/science.1101156View ArticlePubMedGoogle Scholar
- Schrader S, Kaldenhoff R, Richter G: Expression of novel genes during somatic embryogenesis of suspension-cultured carrot cells ( Daucus carota ). J Plant Physiol 1997, 150: 63–68.View ArticleGoogle Scholar
- Li HY, Guo ZF, Zhu YX: Molecular cloning and analysis of a pea cDNA that is expressed in darkness and very rapidly by gibberellic acid. Mol Gen Genet 1998, 259: 393–397. 10.1007/s004380050828View ArticlePubMedGoogle Scholar
- Johnson EP, Mincer T, Schwab H, Burgin AB, Helinski DR: Plasmid RK2 ParB protein: purification and nuclease properties. J Bacteriol 1999, 181: 6010–6018.PubMed CentralPubMedGoogle Scholar
- Bork P, Doolittle R: Drosophila kelch motif is derived from a common enzyme fold. J Mol Biol 1994, 236: 1277–1282. 10.1016/0022-2836(94)90056-6View ArticlePubMedGoogle Scholar
- Adams JC, Kelso R, Cooley L: The kelch repeat superfamily: propellers of cell function. Trends Cell Biol 2000, 10: 17–24. 10.1016/S0962-8924(99)01673-6View ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997, 25: 3389–3402. 10.1093/nar/25.17.3389PubMed CentralView ArticlePubMedGoogle Scholar
- Eddy SR: Profile hidden Markov models. Bioinformatics 1998, 14: 755–763. 10.1093/bioinformatics/14.9.755View ArticlePubMedGoogle Scholar
- Rost B: PHD: predicting one-dimensional protein structure by profile-based neural networks. Methods Enzymol 1996, 266: 525–539.View ArticlePubMedGoogle Scholar
- McGuffin LJ, Bryson K, Jones DT: The PSIPRED protein structure prediction server. Bioinformatics 2000, 16: 404–405. 10.1093/bioinformatics/16.4.404View ArticlePubMedGoogle Scholar
- Kumar S, Tamura K, Jakobsen IB, Nei M: MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 2001, 17: 1244–1245. 10.1093/bioinformatics/17.12.1244View ArticlePubMedGoogle Scholar
- Schultz J, Milpetz F, Bork P, Ponting CP: SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci USA 1998, 95: 5857–5864. 10.1073/pnas.95.11.5857PubMed CentralView ArticlePubMedGoogle Scholar
- Letunic I, Copley RR, Schmidt S, Ciccarelli FD, Doerks T, et al.: SMART 4.0: towards genomic data integration. Nucleic Acids Res 2004, 32: D142–144. 10.1093/nar/gkh088PubMed CentralView ArticlePubMedGoogle Scholar
- Notredame C, Higgins DG, Heringa J: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol 2000, 302: 205–217. 10.1006/jmbi.2000.4042View ArticlePubMedGoogle Scholar
- Goodstadt L, Ponting CP: CHROMA: consensus-based colouring of multiple alignments for publication. Bioinformatics 2001, 17: 845–846. 10.1093/bioinformatics/17.9.845View ArticlePubMedGoogle Scholar
- Rost B, Sander C, Schneider R: PHD – an automatic mail server for protein secondary structure prediction. Comput Appl Biosci 1994, 10: 53–60.PubMedGoogle Scholar
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