Volume 17 Supplement 4
Geena 2, improved automated analysis of MALDI/TOF mass spectra
© Romano et al. 2016
Published: 2 March 2016
Mass spectrometry (MS) is producing high volumes of data supporting oncological sciences, especially for translational research. Most of related elaborations can be carried out by combining existing tools at different levels, but little is currently available for the automation of the fundamental steps.
For the analysis of MALDI/TOF spectra, a number of pre-processing steps are required, including joining of isotopic abundances for a given molecular species, normalization of signals against an internal standard, background noise removal, averaging multiple spectra from the same sample, and aligning spectra from different samples.
In this paper, we present Geena 2, a public software tool for the automated execution of these pre-processing steps for MALDI/TOF spectra.
Geena 2 has been developed in a Linux-Apache-MySQL-PHP web development environment, with scripts in PHP and Perl. Input and output are managed as simple formats that can be consumed by any database system and spreadsheet software. Input data may also be stored in a MySQL database. Processing methods are based on original heuristic algorithms which are introduced in the paper.
Three simple and intuitive web interfaces are available: the Standard Search Interface, which allows a complete control over all parameters, the Bright Search Interface, which leaves to the user the possibility to tune parameters for alignment of spectra, and the Quick Search Interface, which limits the number of parameters to a minimum by using default values for the majority of parameters.
Geena 2 has been utilized, in conjunction with a statistical analysis tool, in three published experimental works: a proteomic study on the effects of long-term cryopreservation on the low molecular weight fraction of serum proteome, and two retrospective serum proteomic studies, one on the risk of developing breat cancer in patients affected by gross cystic disease of the breast (GCDB) and the other for the identification of a predictor of breast cancer mortality following breast cancer surgery, whose results were validated by ELISA, a completely alternative method.
Geena 2 is a public tool for the automated pre-processing of MS data originated by MALDI/TOF instruments, with a simple and intuitive web interface. It is now under active development for the inclusion of further filtering options and for the adoption of standard formats for MS spectra.
KeywordsComputational Proteomics Computational Mass Spectrometry MALDI/TOF mass spectrometry MS spectra comparison/alignment Automation of data analysis Web application LAMP
Mass spectrometry (MS), a still fast improving technology, produces a high volume of data. Large interest therefore exists for MS data management and analysis, and tools are continuously developed to this aim.
In most cases, data are pre-processed at level of instrumentation (including both the instrument itself and the proprietary software that is distributed along with it) for noise subtraction, isotope abundance evaluation, range selection, normalization against a standard signal, and so on. Spectra are then saved for subsequent analysis.
However, the pre-processing is usually performed by applying different conditions, and adaptability of these steps would be useful. Moreover, human controlled re-application of pre-processing procedures on a large number of technical and/or experimental replicates becomes a recurring task, long and potentially source of errors .
It is also noteworthy that the analysis of the final spectrum can change depending on the experimental design. Indeed, when dealing with experimental replicates, it is of interest to generate an average spectrum, as recently demonstrated . Furthermore, the spectra collected for different samples can be compared, in order to highlight similar signals or, on the contrary, dissimilarities between spectra.
The observation of common signals, or the search for distinguishing signals, in other words the search of biomarker signals, has been based on the comparison of mass spectra, and it has been used to classify patients’ samples [1, 3–5], to distinguish microorganisms and animal species [6–9], to detect specific neuropeptides . As these studies demonstrate, the collection of large number of spectra, and their analysis by averaging of replicates and the comparison of the average spectra, is a common practice that represents a field of development for bioinformatics tools.
While many tools exist for MS data management and analysis, allowing most of these elaborations to be carried out by combining their use at different levels, little is currently available for the automation of the fundamental steps involved in the analysis of m/z and abundance data from MS experiments. In fact, it is often reported in literature, and in the previously cited studies, the practice of using single tools together with some manual steps of integration based on Excel data sheets or original scripts. The only alternative can be found by commercial packages, mostly distributed by the manufacturers of spectrometers. Therefore, we decided to develop a free, simple and dedicated MALDI/ TOF (Matrix Assisted Laser Desorption Ionization / Time Of Flight) MS data management and analysis tool, to be available to researchers by a web interface, able to provide an automatic pipeline of analysis of mass spectra, useful to compare them within proteomic studies on large numbers of samples.
Aim of this work
in each spectrum, molecules may be represented by several signals, each corresponding to a different isotopic abundance: these can be summed up to give a total abundance value for each molecule;
often, experimental data have to be normalized against an internal standard in order to obtain (semi) quantitative results;
since experimental data are affected by background noise, the selection of signals above a threshold modulated on the spectra profile may be useful;
the analysis of sample replicates yields multiple spectra which are different because of marginal errors/changes in the experimental phase only: an average spectrum representative of the sample may be determined by aligning these spectra along the m/z axis and computing mean intensity values from the corresponding abundances;
the alignment along the m/z axis may be useful also for comparing spectra obtained from different samples, which could lead to the identification of significant differences in abundances and/or missing signals.
On the basis of these assumptions, we first developed Geena, a prototype tool for filtering, averaging and aligning MALDI/TOF spectra . Geena main limitations are related to: i) the web interface, which is rather complex, since it includes all elaboration parameters in a single form, ii) the type and format of the output, which is also complex and difficult to re-use, and iii) the performances, which are especially poor when the number of spectra involved in the analysis is great.
We present here Geena 2, a redesigned, revised, more performing and much more user-friendly version of Geena.
Work and data flow of the system
In differential proteomics, it is common that two or more groups of samples are compared in order to identify differences, e.g., case samples (patients affected by a given pathology) against control samples (unaffected persons). For each of these groups, a variable number of samples can be made available. In other situations, grouping of samples is not available a priori, and a classification of the samples is instead investigated.
In both cases, a variable number of spectra (replicates) is generated for each sample. This is where the automatic procedure implemented by Geena can be usefully adopted. Replicates may be pre-processed, as described in the following Algorithms sub-section, and “averaged”, thus producing an “average spectrum” which is representative of the sample. Finally, these average spectra can be aligned. The alignment is the basis for any subsequent analysis, e.g. case–control comparison, classification, or biomarker discovery, according to data provided in input.
We developed and made available Geena 2 by using the Linux-Apache-MySQL-PHP (LAMP) web development framework, which is an easy to manage, effective working environment, on a cloud based virtual server, currently with a configuration consisting in a quad core AMD64 2 GHz CPU with 7 Gb RAM memory. The operating system presently is Ubuntu Server 14.04.3 LTS. Current Apache, PHP and MySQL versions respectively are 2.4.7, 5.5.9 and 5.5.47, which are provided by the Ubuntu distribution.
All code originally written for Geena has been revised, corrected and partially rewritten for sake of performances and because of changes introduced in the interfaces, as well as in the output format. Geena 2 is written in PHP , but for the spectra alignment which is instead written in Perl . In more detail, the alignment of spectra is performed with a local run of the NEAPOLIS tool , which uses an original procedure to compare spectra, based on the sorting of all m/z values from spectra under analysis, and the alignment of the values included within an user-defined range of m/z. The aligned signals are then used to generate an average spectrum, and mean and standard deviation are computed for m/z and intensity values. Details about the alignment procedure are available at the NEAPOLIS web site.
Input spectra are managed as tab or comma separated values (respectively, TSV and CSV) text files. These formats can easily be produced by the majority of spectrometers associated software and consumed by any database system and spreadsheet software. For the analysis of the same data with variable parameters, input data may also be stored on our server in a MySQL  database and reused, although this feature is only presently available upon collaboration with the developers. The format of the input is described below, while the Additional file 1 includes a full example input file that can be used for testing purposes.
the original spectrum, as a list of peak pairs representing the m/z and abundance values of the peak, named the “Original” file,
the filtered spectrum, as a list of peak pairs as above, including only peaks selected after the pre-processing analysis, named the “Filtered” file,
information on grouping of isotopic replicas, including lists of selected peaks, each with the list of peak pairs from the original spectrum that were grouped together, named the “Isotopic groups” file.
For each group of replicate spectra from the same sample, Geena 2 output also includes the “average spectrum”, achieved by aligning and averaging all filtered spectra from the same sample, together with related alignment information, which are provided both as text file, for further processing, and as HTML file, for better readability. The average spectrum includes (m/z, abundance) pairs which are computed as average of respective m/z and abundance values for equivalent signals found in the filtered spectra.
In this file, which is meant to be passed on to other software tools for further analysis, values are separated by a tabulation character, and this can limit its readability. Users should better see the equivalent HTML file. The first column reports the number of spectra that were found to include the given peak. From the second to the fifth column, m/z values are shown, the first value being the average one. Empty values correspond to missing peaks in the corresponding spectrum. From the sixth to the last column, abundance values are reported with the same format of m/z values.
The processing methods are based on original heuristic algorithms.
- a.pre-processing of spectra replicates, which in turn consists in:
isotopic peaks identification and joining: signal peaks which are separated by 1 m/z unit (Dalton) in mono-charged species may represent the same molecules, with different isotopic composition, if and only if their abundance values follow the expected distribution, which is variable according to the m/z values of involved peaks. For low mass peptides, the mono-isotopic species largely is more represented than others. At greater masses, non mono-isotopic species gain a greater relevance, having peaks which may become higher than the mono-isotopic one. While the m/z value at which the second species is higher than the first one can only be determined when the exact peptide composition is known, we have experimentally verified by using the Molecular Weight Calculator software  that this usually happens for peptides of molecular weight higher than 1,700 Da. When at least two peaks are recognized as representative of the same molecular species, their abundances are summed up and assigned to the first peak in the series, the monoisotopic one, while the other peaks are removed. Two parameters, namely “Maximum number of isotopic peaks” and “Maximum delta between isotopic peaks”, are available to make this identification and joining tuneable according to the instrument used for generating the spectra. Joining information is provided as output, for verification, in HTML;
normalization: a simple normalization of abundance values is performed by assuming 100.00 the value of a given normalization peak, if available, in order to improve the estimate of the abundances and making them comparable in different acquisitions;
peak selection: a threshold line can be built and only signal peaks above it are selected. The line is built by defining some reference threshold values at given m/z values, including the lowest and highest m/z values for the spectra, and by linearly interpolating between them. Up to six reference values can presently be defined, thus allowing creating a threshold line able to adapt to the shape of the spectra under analysis.
computing average spectra for replicates: spectra which are generated by analysing the same sample can be “averaged” in order to remove single analysis artefacts and strengthen the quality and representativeness of the resulting spectrum.
For this, “equivalent” signals, that is signals that are supposed to represent the same molecular species in original spectra, are first identified by selecting the nearest peaks among those having a difference in m/z values below a given threshold parameter (“Maximum delta for aligning replicates”). If equivalents peaks for a signal are present for a number of spectra which is higher than a given parameter (“Minimum number of signals in replicates”), then both the m/z and abundance average values are computed and assigned to a peak of the average spectrum.
alignment of average spectra: average spectra which are generated by averaging replicated spectra from the same sample, can be aligned in order to highlight differences in signals among samples.
In this case too, equivalent signals, that are supposed to represent the same molecular species in average spectra, are first identified by selecting the nearest peaks among those having a difference in m/z values below a given threshold parameter (“Maximum delta for aligning average spectra”). If equivalents peaks for a signal are present for a number of spectra which is higher than a given parameter (“Minimum number of signals in average spectra”), then both the m/z and abundance average values are computed and assigned to a peak of the aligned spectrum.
Contrary to its predecessor, Geena 2 is available on-line for public access through three simple and intuitive web interfaces. The Standard Search Interface (SSI) allows the user a complete control over all parameters and it is of special interest for expert users and for users with special needs. A simplified interface, named Bright Search Interface (BSI) is also available. It allows users to control both average and alignment parameters, while filter parameters are taken by default. Finally, the Quick Search Interface (QSI) allows users to run Geena 2 by using only a few parameters: the analysis range in m/z and the mass of the normalization peak, if applicable. In this case, default values are used for the majority of parameters.
The second section (“Input data”) is meant to define data input method and to specify data to be analysed. Two methods are available: file upload and selection of data from a local database. The second option is only available for collaborating parties. It may be especially useful for repeated analysis with variable parameters. The third section (“Filter and isotopic peaks join”) allows specifying in detail the requested pre-processing tasks and the related parameters. It allows defining the analysis range in Dalton by defining minimum and maximum m/z values to be taken into account. It also supports the definition of the presence of a normalization peak and of its m/z value in Da. The linear filter, when desired, is also specified in this section by introducing the threshold values at various m/z. Finally, the parameters related to the optional joining of isotopic peaks can also be specified in this section.
The fourth section (“Computation and alignment of average spectra“) allows to set the parameters for averaging the replicates and for generating a final alignment and an average spectrum for all spectra under analysis.
Only signals present in all spectra
Signals present in all spectra but one
Signals present in the majority of spectra (>50 %)
All signals present in at least two spectra
All signals, even when present in one spectra only
Signals present in at least a number of spectra specified by the user
A high number of spectra presenting the signal is preferable for the averaging process, which determines the average spectrum of spectra replicates for the same sample. In this case, only those signals that are present in many, if not all, spectra is included in the average spectrum, thus avoiding to include sporadic signals, possibly arising from the spectrum acquisition process. On the contrary, a low number of signals is better than a high one for the alignment process, where average spectra from distinct samples are compared. In this case, signals are included in the alignment even when they are rare, thus facilitating and empowering the following differential analysis.
Intermediate outputs contain filtered MS spectra and their alignments. All results are available for downloading from the result page and a link to a compressed archive is also available in the results page and sent by email.
Validation and application examples
In the following, we present three examples of experiments where the analysis of MS spectra generated with a MALDI/TOF instrument was carried out by using either Geena 2 or its previous releases.
First, we carried out a proteomic study on the effects of long-term cryopreservation on the low molecular weight fraction of serum proteome (3). Blood samples used in this study either were stored at −20 °C for 8 years after previously being collected from 17 healthy donors or were freshly collected from 13 other unrelated healthy donors. From the freshly collected samples, two sets of aliquots were generated. One set was stored at −80 °C and analysed 18 months later, whereas eight samples of the second set were immediately subjected to analysis. Raw data generated by MALDI/TOF analysis within a dynamic m/z range from 800 to 2,500, were exported as an Excel spreadsheet. Data handling was performed using “PROTEO” and “NEAPOLIS”, two bioinformatic tools that were later merged and formed Geena. PROTEO allowed isotopic peaks joining, normalization, and peak selection, while NEAPOLIS was used both to obtain the mean abundance values for experimental replicates and to align mass spectra along the m/z axis. Statistical analysis performed on data elaborated by NEAPOLIS generated a panel of 106 m/z values whose abundances were significantly different between the experimental groups.
Geena was for the first time employed in a retrospective proteomic study on cryopreserved sera from patients affected by gross cystic disease of the breast (GCDB), a common benign disease of the mammary gland, affecting some 7 % of women in Western countries . For the realization of this study two experimental groups were established: a) thirty women who developed a breast cancer following first cyst aspiration served as cases, b) sixty women who, by the same date limit, were still breast cancer free served as controls. Each sample was analysed in quadruplicate by MALDI/TOF MS. Raw data were pre-processed by Geena which generated a panel of 96 candidate signals to be subjected to statistical analysis. The following Significance Analysis of Microarray (SAM), performed by taking as input the results provided by Geena, identified a significant increase in serum levels of complement fraction C3f in GCDB patients who during the follow-up developed a breast cancer.
Recently, the combined utilization of Geena 2 and SAM allowed the identification of angiotensin II serum level as a predictor of breast cancer mortality following breast cancer surgery . In this case, an independent validation of Geena 2 data processing has been obtained by using the alternative bio-analytical technique ELISA immunoassay, which confirmed the same results.
Despite the fact that in many applications the peak selection can be achieved by means of the Geena 2 linear filter described in the previous section, noisy datasets might demand a more accurate treatment. In the works reported above an alternative external filtering tool, a stand-alone National Instruments LabVIEW  program named MS-BASELINER was used. MS-BASELINER is able to dynamically evaluate data background along the spectrum, discriminating between spurious, noisy peaks and real peaks, through an iterative process which determines a background noise value for each peak in the spectrum by analysing all peaks around it only. MS-BASELINER can be downloaded from the Geena 2 website along with a detailed description .
Comparison with existing tools
Despite the existence of many bioinformatic tools covering the main aspects of proteomics investigation, the lack of online tools suitable for creating an archive of MALDI MS spectra and to analyse-reanalyse them with different parameters makes Geena unique under this aspect. Concerning the single steps of analysis performed, there are tools available for pre-processing of MS spectra within the MatLab bioinformatics tool box as well as in R, two development environments largely used by bioinformatics, but, in our experience, not simple to use for biochemists and MS analysts. On the other hand, tools for aligning spectra are available within the same MatLab and R environments, as well as in form of independent tools [20, 21]. The unique feature of Geena 2, however, is its workflow architecture, that makes it possible to integrate other tools for each step of the analysis, leaving into the interface the opportunity to select the most suitable to the user work. As further development, the integration of freely available tools for the pre-processing, filtering, and aligning of spectra will be considered to make the tool even more flexible and useful to a wider community.
Elaborating from previous tools PROTEO, NEAPOLIS, and Geena, the original software Geena 2 can now be considered a useful public-domain, free tool for the automated pre-processing of relatively uncomplicated MS data, like those originated by MALDI/TOF instruments. Its flexible, yet simple and intuitive web interface, as well as the simple layout of the results, makes it very powerful, yet easy to use even for not particularly experienced operators. Its output can straightforward be used as input for further analysis, including differential analysis and biomarker discovery, by using many publicly available software tools.
It has proven to be useful and effective for published scientific works, where it has been adopted for pre-processing of MALDI/TOF spectra which were then compared by using statistical software on a limited amount of data.
The tool is publicly available on-line and it can also be distributed on collaborations.
Current developments of Geena 2 include an extension of its pre-processing, filtering, and aligning features and of accepted input and output formats.
As discussed, the MS-BASELINER filter has been adopted for a more sophisticated identification of background noise. A new version of this tool is being implemented within Geena 2 and will be optionally available soon. Other filtering methods are under evaluation. Since mzML  is now a standard format for archiving and exchanging MS spectra, Geena 2 will soon adopt it for input data.
Further developments under evaluation include parallelization of alignment tasks and the creation of user-specific environments accessible after user authentication allowing both the upload of confidential data in a controlled access table of the database and the definition of preferred parameters for processing and alignment of spectra.
Availability and requirements
Project name: Geena 2
Project home page: http://bioinformatics.hsanmartino.it/geena2/
Operating system(s): Ubuntu 14.04.3 LTS
Programming languages: LAMP Framework, PHP and Perl
Other requirements: Apache HTTPD, MySQL
License: no license defined, software available on collaborations
Any restrictions to use by non-academics: none
advanced micro devices
bright search interface
central processing unit
comma separated values
gross cystic disease of the breast
HyperText Markup Language
matrix assisted laser desorption ionization time-of-flight
mass spectrometry markup language
PHP: Hypertext Preprocessor (recursive definition, originally “Personal Home Page”)
quick search interface
random access memory
significance analysis of microarray
standard search interface
tab separated values
AF activity has been supported by the Flagship “InterOmics” project (PB.P05) funded by the Italian Ministry of Education, University and Research and Italian National Research Council organizations. This work was supported by grants from Italian Ministry of Health (5 x 1000 funds 2013).
Publication charges for this article have been funded by the IRCCS AOU San Martino IST institute on its own funds.
This article has been published as part of BMC Bioinformatics Volume 17 Supplement 4, 2016. Selected works from the 14th International Workshop on Network Tools and Applications in Biology (NETTAB 2014). The full contents of the supplement are available online at https://bmcbioinformatics.biomedcentral.com/articles/supplements/volume-17-supplement-4.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- D’Imperio M, Della Corte A, Facchiano A, Di Michele M, Ferrandina G, Donati MB, et al. Standardized sample preparation phases for a quantitative measurement of plasma peptidome profiling by MALDI-TOF. J Proteomics. 2010;73(7):1355–67. doi:10.1016/j.jprot.2010.03.001. PMID: 20227539.View ArticlePubMedGoogle Scholar
- Mitchell M, Mali S, King CC, Bark SJ. Enhancing MALDI time-of-flight mass spectrometer performance through spectrum averaging. PLoS One. 2015;10(3):e0120932. doi:10.1371/journal.pone.0120932. PMID: 25798583.View ArticlePubMedPubMed CentralGoogle Scholar
- Mangerini R, Romano P, Facchiano A, Damonte G, Muselli M, Rocco M, et al. The application of atmospheric pressure MALDI to the analysis of long-term cryopreserved serum peptidome. Anal Biochem. 2011;417(2):174–81. doi:10.1016/j.ab.2011.06.021. PMID: 21756868.View ArticlePubMedGoogle Scholar
- Profumo A, Mangerini R, Rubagotti A, Romano P, Damonte G, Guglielmini P, et al. Complement C3f serum levels may predict breast cancer risk in women with gross cystic disease of the breast. J Proteomics. 2013;85:44–52. doi:10.1016/j.jprot.2013.04.029. PMID: 23639844.View ArticlePubMedGoogle Scholar
- Siciliano RA, Mazzeo MF, Spada V, Facchiano A, D’Acierno A, Stocchero M, et al. Rapid peptidomic profiling of peritoneal fluid by MALDI-TOF mass spectrometry for the identification of biomarkers of endometriosis. Gynecol Endocrinol. 2014;30(12):872–6. doi:10.3109/09513590.2014.943718. PMID: 25111755.View ArticlePubMedGoogle Scholar
- Fang J, Dorrestein PC. Emerging mass spectrometry techniques for the direct analysis of microbial colonies. Curr Opin Microbiol. 2014;19:120–9. doi:10.1016/j.mib.2014.06.014. PMID: 25064218.View ArticlePubMedGoogle Scholar
- Mazzeo MF, Sorrentino A, Gaita M, Cacace G, Di Stasio M, Facchiano A, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the discrimination of food-borne microorganisms. Appl Environ Microbiol. 2006;72(2):1180–9. doi:10.1128/AEM.72.2.1180-1189.2006. PMID: 16461665.View ArticlePubMedPubMed CentralGoogle Scholar
- Mazzeo MF, De Giulio B, Guerriero G, Ciarcia G, Malorni A, Russo GL, et al. Fish authentication by MALDI-TOF mass spectrometry. J Agric Food Chem. 2008;56(23):11071–6. doi:10.1021/jf8021783. PMID: 19007297.View ArticlePubMedGoogle Scholar
- Dhieb C, Normand AC, L’Olliver C, Gautier M, Vranckx K, El Euch D, et al. Comparison of MALDI-TOF mass spectra with microsatellite length polymorphisms in Candida albicans. J Mass Spectrom. 2015;50(2):371–7. doi:10.1002/jms.3538. PMID: 25800019.View ArticlePubMedGoogle Scholar
- Salisbury JP, Boggio KJ, Hsu YW, Quijada J, Sivachenko A, Gloeckner G, et al. A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics. Mol Brain. 2013;6:60. doi:10.1186/1756-6606-6-60. PMID: 24373546.View ArticlePubMedPubMed CentralGoogle Scholar
- Romano P, Profumo A, Mangerini R, Ferri F, Rocco M, Boccardo F, et al. Geena, a tool for MS spectra filtering, averaging and aligning. EMBnet J. 2012;18(A):125–6. 10.14806/ej.18.A.454.View ArticleGoogle Scholar
- PHP: Hypertext Preprocessor. http://php.net/. Accessed 1 February 2016.
- The Perl Programming Language. http://www.perl.org/. Accessed 1 February 2016.
- NEAPOLIS. http://bioinformatica.isa.cnr.it/NEAPOLIS/. Accessed 1 February 2016.
- MySQL :: The world’s most popular open source database. http://www.mysql.com/. Accessed 1 February 2016.
- Molecular Weight Calculator for Windows. http://www.alchemistmatt.com/mwtwin.html. Accessed 1 February 2016.
- Boccardo F, Rubagotti A, Nuzzo PV, Argellati F, Savarino G, Romano P, et al. Matrix-assisted laser desorption/ionisation (MALDI) TOF analysis identifies serum Angiotensin II concentrations as a strong predictor of all-cause and breast cancer (Bca)-specific mortality following breast surgery. Int J Cancer. 2015;137(10):2394–402. doi:10.1002/ijc.29609. PMID: 25994113.View ArticlePubMedGoogle Scholar
- LabVIEW System Design Software - National Instruments. http://www.ni.com/labview/. Accessed 1 February 2016.
- Geena 2: MS-BASELINER. http://bioinformatics.hsanmartino.it/geena2/background.php. Accessed 1 February 2016.
- Tang Z, Zhang L, Cheema AK, Ressom HW. A new method for alignment of LC-MALDI-TOF data. Proteome Sci. 2011;9 Suppl 1:10. doi:10.1186/1477-5956-9-S1-S10. PMID: 22166061.View ArticleGoogle Scholar
- Wong JW, Cagney G, Cartwright HM. SpecAlign – processing and alignement of mass spectra. Bioinformatics. 2005;21(9):2088–90. doi:10.1093/bioinformatics/bti300. PMID: 15691857.View ArticlePubMedGoogle Scholar
- mzML 1.1.0 Specification | HUPO Proteomics Standards Initiative. http://www.psidev.info/mzml_1_0_0. Accessed 1 February 2016.