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Table 1 Optimal production of the twelve biosynthetic precursors and the metabolic cofactors.

From: Metabolic flux balance analysis and the in silico analysis of Escherichia coli K-12 gene deletions

Compound μmol/g DW Compound μmol/g DW
Amino Acids   Phospholipids  
Alanine 488 Phosphatidyl serine 2.58
Arginine 281 Phosphatidyl ethanolamine 96.75
Asparagine 229 Phosphatidyl glycerol 23.22
Aspartate 229 Cardiolypin 6.45
Cysteine 87 Fatty acid composition (% of total fatty acid)  
Glutamate 250 Myristic acid (2.68)  
Glutamine 250 Myristoleic acid (7.70)  
Glycine 582 Palmitic acid (38.23)  
Histidine 90 Palmitoleic acid (10.74)  
Isoleucine 276 Heptadecenoic acid (16.11)  
Leucine 428 cis-Vaccenic acid (0.90)  
Lysine 326 Oleic acid (17.91)  
Methionine 146 Nonadecenoic acid (5.73)  
Phenylalanine 176 Cell Wall Sructures  
Proline 210 Lipopolysaccharide 8.4
Serine 205 Peptidoglycan 27
Threonine 241 Cofactors and other molecules  
Tryptophan 54 5-Methyl-THF 50.0
Tyrosine 131 Putrescine 35.0
Valine 402 Spermidine 7.0
Protein synthesis/processing (ATP/ Amino Acid) 4.306 NAD 2.15
Ribonucleotides   NADH 0.05
ATP 165 NADP 0.13
GTP 203 NADPH 0.4
CTP 126 UDP-Glucose 3.0
UTP 136 ATP 4.0
RNA synthesis/processing (ATP/Nucleotide) 0.40 ADP 2.0
Deoxyribonucleotides   AMP 1.0
dATP 24.7 CoA 0.03
dTTP 24.7 Acetyl-CoA 0.04
dGTP 25.4 Succinyl-CoA 0.01
dCTP 25.4 Glycogen 154
DNA synthesis/processing (ATP/Nucleotide) 1.372   
  1. The optimal production was calculated for the wild-type strain and the deletion strains. The constraints are set as defined in Figure 1. The color code quantitatively defines effect of the in silico deletion; red corresponds to 0.0, yellow corresponds to 0-50% of the wild-type production, blue corresponds to 50-100% of the wild-type, and no color coding is illustrated when the production is unchanged from the wild-type. Data for other carbon sources is available online. G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; R5P, ribose-5-phosphate; E4P,erythrose 4-phosphate; T3P1, glyceraldehyde 3-phosphate; 3PG, 3-phosphoglycerate; PEP, phosphoenolpyruvate; PYR, pyruvate; ACCOA, acetyl-CoA; AKG, α-ketoglutarate; SUCCOA, succinyl-CoA; OA, oxaloacetate.