Figure 2
From: Island method for estimating the statistical significance of profile-profile alignment scores
Change in E-value as a function of variance in λ. Impact of variance in λ on E-values for marginally significant alignment scores (p-value ≈ 10-9) between profiles of lengths 350. For example, 1%, 3%, and 5% error in lambda leads to an error in E-value by a factor greater than 1.3, 2.3, and 4, respectively. On the other hand, 20% change in λ leads to an almost 300 fold change in the estimated E-value.