Skip to main content
Figure 5 | BMC Bioinformatics

Figure 5

From: Robust MS quantification method for phospho-peptides using 18O/16O labeling

Figure 5

Experimental label swapped spectra pair. Fig. 5 The two label swapped spectra pairs are shown (Direct and Inverted) for the peptide ASGQAFELILp SPR. The observed spectra S (yellow bars) is shown along with the model reconstruction using the three contributing isotopic contours (TLight(orange), TMixed(green) and THeavy(blue)). The labeling efficiency for this particular peptide was rather low: 0.61, but due to the clear signal it was possible to estimate a fold change of 2.49 [2.05 3.21]. The ε/S-ratio was 0.06 reflecting a model fit around average for the experimental spectra. The spectral intensity was found to vary considerably between the direct and inverted label swapped experiments (average peak intensities: <SD> = 1899 and <SI> = 7880), which is likely to have originated primarily from variation in phospho-peptide enrichment.

Back to article page