Figure 1

Workflow of OrthoSelect. The main workflow of the software pipeline to detect ortholog sequences in phylogenomic studies. Input are EST libraries and an ortholog database (either KOG or OrthoMCL) as multi-fasta files. The analysis comprises four parts. (1) The orthology detection – which can be performed on a single computer or a computer cluster – blasts each EST against the ortholog database, selects the closest ortholog group as the best hit and translates it and stored together with the nucleotide sequences in the corresponding OG. (2) Target genes can be selected. (3) The sequence most likely being an ortholog is selected by eliminating potential paralogs. (4) Informative alignment columns are selected to increase the phylogenetic signal.