From: QPCR: Application for real-time PCR data management and analysis
Feature | QPCR | StatMiner 3.0 (Integromics) [32] | qBase 1.3.5 [5] | qBasePlus 1.1 (Biogazelle) [33] | GenEx 4.4.2 (MultiD) [34] | qPCR-DAMS [6] |
---|---|---|---|---|---|---|
Import (Applied Biosystems, Lightcycler, BioRad, Excel) | ✓1/✓2/-/✓ | ✓/-/-/✓ | ✓/✓/✓/✓ | ✓/✓/✓/✓ | -/-/-/✓ | -/-/-/✓ |
Absolute/relative quantification | -/✓ | ✓/✓ | -/✓ | -/✓ | ✓/✓ | ✓/✓ |
RDML compliant | -3 | - | - | ✓ | - | - |
Calculate Cq | ✓ | - | - | - | - | ✓ |
Calculate reaction specific efficiency | ✓ | - | - | - | - | - |
Calculate target specific efficiency | ✓ | ✓ | ✓ | ✓ | ✓ | - |
Includes geNorm, Normfinder | ✓/- | ✓/✓ | ✓/- | ✓/- | ✓/✓ | -/- |
Normalization | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Inter-run calibration | ✓ | - | ✓ | ✓ | - | ✓ |
Calculate fold change ratios | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Quality control | ✓ | ✓ | ✓ | ✓ | - | ✓ |
Statistical tests | ✓ | ✓ | - | - | ✓ | - |
Clustering | - | ✓ | - | - | ✓ | - |
Correlation | - | - | - | - | ✓ | - |
Error propagation | ✓ | - | ✓ | ✓ | - | - |
2D, 3D, Scatter, Bar plots | ✓/-/-/✓ | ✓/-/-/✓ | -/-/-/✓ | -/-/-/✓ | ✓/✓/✓/✓ | -/-/-/- |
Multi-user functionality | ✓ | - | - | - | - | - |
Database storage | ✓ | ✓ | - | - | - | ✓ |
Web/Standalone/Plugin | ✓/-/- | -/✓/- | -/-/✓4 | -/✓/- | -/✓/- | -/-/✓5 |
Freely available | ✓ | - | ✓ | - | - | ✓ |