Figure 2

The diagram depicts how processes and agents are overlaid. Agents are pink, green and yellow, as represented in Fig 1. Arrows represent communication channels between the expert system and the other agents. The persistence agent is not represented as it is used throughout the process: (input). The user gives the two boundary genes of the target region - (1) The region is completed by all genes present in the Ensembl database - (2) The phylogenetic tree is computed for each gene - (3) Orthologous genes are calculated (forester) - (4) Each orthologous gene is located on the chromosome in its species - (5) Orthologous genes are clusterized if they are in the same chromosome and the same species. If the cluster contains fewer than three genes, it is removed - (6) Clusters are completed with genes contained in the region and that are not orthologous genes - (7) A score is calculated for each cluster: if the conserved site is not significant, then the cluster is removed - (8) The system restarts with new regions.