Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum

Table 1 Sub-cellular localisation predictions using WolfPsort of proteins over-represented within the Mascot peptide supported gene annotations of S. nodorum.¹

Localisation	Peptide supported genes (all)	Peptide supported genes (v1)	Peptide supported genes (v2)	Unsupported genes	Expected genes	Total Genes
cytoplasm	605	24	581	2118	361	2723
cytoplasm/nucleus	87	7	80	491	77	578
other	1442	157	1285	11346	1094	12788
unassigned	0	0	0	27	4	27
TOTAL	2134	188	1946	13982	2136	16116

¹Numbers of peptide supported genes in each category were compared to expected counts from a random sampling of the whole genome via Fisher's exact test at a significance threshold of <0.05.

ISSN: 1471-2105