Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum

Table 7 Summary of the 266 6-frame translated genome-mapped peptide clusters¹ not confirming existing S. nodorum CDS annotations by either overlap or proximity within 200 bp.

In conflicting orientation with existing gene annotation	113
No conflict, no supporting evidence	135
Overlaps genomic tblastn hit	18
Genomic tblastn hit links to an existing gene	15
Genomic tblastn hit	3

¹ Peptide clusters were assessed for orientation conflicts with genes in the opposing strand and for overlap with grouped regions of tblastn homology to related dothideomycete genomes (L. maculans, P. tritici-repentis, C. heterostrophus, A. brassisicola, M. graminicola and M. fijiensis).

ISSN: 1471-2105