Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum

Table 8 Summary of 6-frame translated unassembled reads supported by MASCOT peptides.

Unassembled Read Contigs	939
with peptide support	423
with blastx hit to dothideomycetes	270
Hits S. nodorum gene	241
does not hit S. nodorum gene	29
Without blastx hit to dothideomycetes	153
without peptide support	516
Unassembled Read Singletons	10839
with peptide support	651
with blastx hit to dothideomycetes	437
hits S. nodorum gene	422
does not hit S. nodorum gene	15
Without blastx hit to dothideomycetes	214
without peptide support	10188

15455 reads that were not included in the main genome assembly of S. nodorum were re-assessed for overlap. 4616 reads were clustered into 939 contigs, with 10839 singleton reads remaining.

ISSN: 1471-2105