TY - JOUR AU - You, Frank M. AU - Huo, Naxin AU - Gu, Yong Q. AU - Lazo, Gerard R. AU - Dvorak, Jan AU - Anderson, Olin D. PY - 2009 DA - 2009/10/13 TI - ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery JO - BMC Bioinformatics SP - 331 VL - 10 IS - 1 AB - In some genomic applications it is necessary to design large numbers of PCR primers in exons flanking one or several introns on the basis of orthologous gene sequences in related species. The primer pairs designed by this target gene approach are called "intron-flanking primers" or because they are located in exonic sequences which are usually conserved between related species, "conserved primers". They are useful for large-scale single nucleotide polymorphism (SNP) discovery and marker development, especially in species, such as wheat, for which a large number of ESTs are available but for which genome sequences and intron/exon boundaries are not available. To date, no suitable high-throughput tool is available for this purpose. SN - 1471-2105 UR - https://doi.org/10.1186/1471-2105-10-331 DO - 10.1186/1471-2105-10-331 ID - You2009 ER -