Set 1 results under equal target and nontarget concentrations. (a) PICKY-predicted target (green) and nontarget (red) annealing temperatures for the chosen probes. (b-e) Measured T arget and N ontarget intensities of individual probes at different temperatures; the median values among probes at each temperature are shown in the label. (f) Combined target intensities (green), nontarget intensities (red) and their ratios (blue) across all hybridization temperatures. (g-j) Target and nontarget intensity R atios of individual probes at different temperatures; the median values among probes are shown in the labels. In these figures, boxes enclose measured values within the lower and upper quartiles, circled dots indicate median values, whisker lines extending from either end of the boxes show the extent of the rest of data, and isolated empty circles indicate outliers.