Table 1 Oligo dilution levels and experiment design
Set 1 sample oligos | |||||
|---|---|---|---|---|---|
Conc. | E1 | E2 | E3 | E4 | E5 |
10 -5 M | t1 t6 n5 n10 | t5 t7 n4 n6 | t4 t8 n3 n7 | t3 t9 n2 n8 | t2 t10 n1 n9 |
10 -6 M | t2 t7 n4 n9 | t1 t8 n3 n10 | t5 t9 n2 n6 | t4 t10 n1 n7 | t3 t6 n5 n8 |
10 -7 M | t3 t8 n3 n8 | t2 t9 n2 n9 | t1 t10 n1 n10 | t5 t6 n5 n6 | t4 t7 n4 n7 |
10 -8 M | t4 t9 n2 n7 | t3 t10 n1 n8 | t2 t6 n5 n9 | t1 t7 n4 n10 | t5 t8 n3 n6 |
10 -9 M | t5 t10 n1 n6 | t4 t6 n5 n7 | t3 t7 n4 n8 | t2 t8 n3 n9 | t1 t9 n2 n10 |
Set 2 sample oligos | |||||
Conc. | E1 | E2 | E3 | E4 | E5 |
10 -5 M | t1 t6 n1 n6 | t5 t7 n5 n7 | t4 t8 n4 n8 | t3 t9 n3 n9 | t2 t10 n2 n10 |
10 -6 M | t2 t7 n2 n7 | t1 t8 n1 n8 | t5 t9 n5 n9 | t4 t10 n4 n10 | t3 t6 n3 n6 |
10 -7 M | t3 t8 n3 n8 | t2 t9 n2 n9 | t1 t10 n1 n10 | t5 t6 n5 n6 | t4 t7 n4 n7 |
10 -8 M | t4 t9 n4 n9 | t3 t10 n3 n10 | t2 t6 n2 n6 | t1 t7 n1 n7 | t5 t8 n5 n8 |
10 -9 M | t5 t10 n5 n10 | t4 t6 n4 n6 | t3 t7 n3 n7 | t2 t8 n2 n8 | t1 t9 n1 n9 |