Table 3 Barcode sequence space(s) defined by program parameters.
| Â | Parameters | Results | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Run | Len -1 | Min. Dist. -m | GC Low -c | GC High -g | Homo-polymer -p | Hair-pin -a | Hetero-duplex -b | Dels. -d | Initial Barcode Pool | Final Barcode Pool | Exec. Time (sec.) 1 | |
| Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | System A 2 | System B 3 |
1 | 4 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 256 | 7 | 0.0 (0.0) | 0.0 (0.0) |
2 | 5 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 1024 | 26 | 0.0 (0.0) | 0.0 (0.0) |
3 | 6 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 4096 | 82 | 0.4 (0.5) | 0.2 (0.4) |
4 | 7 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 16384 | 218 | 3.0 (0.0) | 1.2 (0.4) |
5 | 8 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 65536 | 760 | 54 (0.0) | 24 (0.4) |
6 4 | 9 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 262144 | 2774 | 978 (0.4) | 419 (5.4) |
7 | 9 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 0 | 262144 | 3113 | 12 (0.0) | 6.0 (0.0) |
8 | 9 | 4 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 262144 | 593 | 159 (0.4) | 68 (0.8) |
9 | 9 | 3 | 0.3 | 0.7 | 4 | 5 | 5 | 1 | 262144 | 3294 | 1536 (0.8) | 655 (5.8) |
10 | 9 | 3 | 0.3 | 0.7 | 3 | 4 | 4 | 1 | 262144 | 2774 | 979 (0.7) | 416 (1.2) |
11 | 9 | 3 | 0.3 | 0.7 | 3 | 6 | 6 | 1 | 262144 | 2774 | 977 (1.1) | 419 (9.3) |
12 | 9 | 3 | 0.4 | 0.6 | 3 | 5 | 5 | 1 | 262144 | 2155 | 493 (0.5) | 211 (0.8) |
13 | 9 | 3 | 0.3 | 0.7 | 2 | 5 | 5 | 1 | 262144 | 779 | 42 (0.4) | 18 (0.4) |
14 | 9 | 3 | 0.2 | 0.8 | 3 | 5 | 5 | 1 | 262144 | 2914 | 1153 (0.8) | 491 (4.9) |
15 | 9 | 3 | 0.1 | 0.9 | 3 | 5 | 5 | 1 | 262144 | 2910 | 1177 (0.0) | 505 (5.5) |
16 | 10 | 3 | 0.3 | 0.7 | 3 | 5 | 5 | 1 | 1.10E+06 | 9375 | 16507 (1857) | 6650 (23) |