Integration of Commercial Tools for multiplierz Reports. A spreadsheet-based report from multiplierz analysis of 11 LC-MS analyses, designed to interrogate performance of LC column geometry and flow rate (see also Figure 5 and Additional File 5). For clarity the spreadsheet shows peptide entries and characterization data from the two extremes in column size and flow rate. Informative images are embedded within spreadsheet cell comments and are accessed by mouse-over, thus facilitating rapid visual inspection. Optional embedded images include: 1.) MS/MS spectra that are annotated with b- and y-type fragment ion labels, peptide sequence, search engine score (in this case Mascot peptide score), and precursor charge state. A color scheme highlights modified amino acids (in this case oxidized methionine) and those residues inferred from b- and y-type fragment ion assignments (horizontal lines, red and blue denote singly- and doubly-charged ions, respectively). 2.) RIC images in which MS scans are annotated with green squares, while yellow circles and blue triangles denote MS/MS scans for the precursor of interest, with the latter indicating the specific MS2 event described in the selected row of the spreadsheet. 3.) precursor region of the MS spectrum (not shown).