Multiplierz -based Workflow for Analysis of LC Column Geometry and Flow Rate. Relative performance improvement for analysis of peptides derived from whole cell lysate as a function of column size and flow rate (a). Original data contained ~90,000 MS/MS scans (b) encompassing almost 23,000 peptide assignments (combination of sequence, charge state, and modification), across 11 LC-MS acquisitions. In addition, multiplierz used Mascot-derived peptide identifications (c) to generate RICs, calculate full chromatographic peak width at half-maximum (FWHM), and determine precursor apex intensity (d). Common peptide sequences and associated analytical metrics are extracted (e) into a final, spreadsheet based report (f).