An example of the prediction results of the target T0409 from the (PS)2-v2 server. The alignment and predicted structure of the BIG_1156.2 domain of putative penicillin-binding protein MrcA from Nitrosomonas europaea ATCC 19718 using the (PS)2-v2 server. (A) The alignment between the query and the selected template, translation initiation factor 5A protein (PDB code 1bkbA), from Pyrobaculum aerophilum. (B) The superposition, the native structure of T0409 (broad, PDB code 3d0f) and the predicted structure (thin). The green blocks are the regions that the predicted structure matches to the native structure. The yellow and purple blocks indicate the shift errors between predicted structure and native structure, the Cα distances between them are <5 Å and >5 Å, respectively.