An experimental loop design for the detection of constitutional chromosomal aberrations by array CGH

BMC Bioinformatics

Table 4 The non-confirmed positives.

Clone	Status	Validation method
RP11-114K7	[24]
RP11-161M6	[24]
RP1-225D2	[24]
RP6-14C6	[24]
RP11-342F21	[24]
RP11-342F21	[24]
RP6-22D12	[24]
RP1-93N13	Genomic Database of Variants
RP11-2P5	Single clone deletion	qPCR
RP11-48E16	Single clone deletion	qPCR
RP11-127A9	Single clone deletion	qPCR
RP11-469N6	Single clone deletion	qPCR
RP11-576I16	Single clone duplication	qPCR
RP11-152O18	Single clone deletion	FISH
RP11-361M10	Not confirmed	Lack of DNA
RP11-404P12	Low spot quality
RP5-982E9	Low spot quality
RP11-301H15	Low spot quality
RP4-742J24	Low spot quality

Outside the duplicated and deleted regions, 15 reporters were also classified as being completely duplicated or deleted. Eight of them could be confirmed as being polymorphic clones according to [24] and the Genomic Database of Variants. Six of them confirmed with FISH or qPCR. One clone could not be confirmed, due to lack of DNA.

ISSN: 1471-2105