Skip to main content


Figure 2 | BMC Bioinformatics

Figure 2

From: siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect

Figure 2

Screenshots from siDirect 2.0 webserver. (A) A typical output of siDirect 2.0: siRNAs targeting human interferon β-1 (NM_002176) are designed. (B) By clicking the individual siRNA in (A), a detailed list of off-target gene candidates with near-perfect matches is displayed separately for each siRNA strand. The alignment between each off-target sequence and the siRNA sequence clearly visualizes the positions of mismatches.

Back to article page