Figure 2
From: Using mechanistic Bayesian networks to identify downstream targets of the Sonic Hedgehog pathway
Comparing the relative gene expression profile of 8 selected putative targets. From these expression patterns one can see that the profiles for Gas1 and Gli1 closely follow changes in Shh and Ptch1 expression. Gli2 and Gli3 show higher activation in the adult tissues as compared to the somite samples but, even in the adult tissues, their expression pattern does not correspond to the knockout state of Shh. Foxa2 expression shows a strong response to the Ptch1 knockouts in the somite samples and with Shh knockouts in the adult head tissues but there is no pattern in the adult limb and trunk tissues. While the patterns in Gli2, Gli3, Foxa2 are significant, the patterns either do not coincide with patterns in the early steps of the Shh pathway (Shh, Ptch1, Smo) indicating that there may be other genes involved in their regulatory control or the patterns do not hold over all tissues. Accordingly, those genes do not score highly in the MBN method.