Figure 5

Relative nucleosome preference of different subsets of synthetic 150-mers. (A) and (B) Dependence of relative nucleosome preference (as log₂(occupancy ratio)) on G+C content (A) and maximum poly-A length (B). Oligonucleotides categorized as "Neutral %G+C" in (B) are those with 45-55% G+C. Graph below shows the frequency of the selected attribute in the oligonucleotides analyzed, and also the human and yeast genomes. (C) Dependence of relative occupancy on poly-A content and CpG status. Poly-A containing oligonucleotides are defined as containing at least four consecutive adenine bases. CpG oligonucleotides are defined as having a G+C content ≥50%, with an observed/expected CpG ratio ≥0.6 (Obs/Exp CpG = Number of CpG * N/(num G * num C), where N = length of sequence[37]). The sequencing readout (rather than array readout) data from the Kaplan paper was used in this analysis. On all box plots, whiskers indicate 10^th and 90^th percentiles.