Figure 2

Yeast two-hybrid confirmation of a predicted interaction. AtGluRS interacts with a disease resistance protein (DRP), glutamine synthetase (GS) and a zinc finger protein (ZFP). Three independent AH109 transformants were spotted onto yeast minimal plates lacking leucine and tryptophan (-LW) to select for the prey and bait plasmids respectively, or lacking histidine and adenine (-LWAH) to indicate reporter gene activation. Assays use a Gal4 based yeast two-hybrid system.