Figure 4

A peptide highlights a missing splice form. Part of the gene model of the Na pump alpha subunit (Atpα, CG5670) is depicted. In front of the black background, different types of sequence data are displayed: several predictions (light pink, purple, and different shades of turquoise), conserved coding regions (bright yellow), cDNAs alignments (greens), and peptides from the PeptideAtlas (bright pink). In front of the light blue background, alternative splice forms annotated in release 5.12 are shown in dark blue. The PeptideAtlas peptide PAp00073066 was identified in a 6-frame search and maps within the Atpα gene region. Note that while prediction algorithms postulate an alternative exon in this region, there are no supporting cDNAs (nor ESTs; not shown). The splice variant Atpα-PI, added in FlyBase annotation release 5.11, now accounts for the identified peptide sequence, NPEIDNLVNER. The codon for the last residue of the peptide spans the adjacent intron, thus supporting the annotated splice sites.