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Table 1 Experimentally validated PHA depolymerases, which were used as seed sequences to set up the DED.

From: The PHA Depolymerase Engineering Database: A systematic analysis tool for the diverse family of polyhydroxyalkanoate (PHA) depolymerases

Accession number (gi) Organism Family Reference
3641686 Ralstonia eutropha H16 Intracellular nPHASCL depolymerases (no lipase box) [28]
75763431 Bacillus thuringiensis serovar israelensis ATCC 35646 Intracellular nPHASCL depolymerases (lipase box) [35]
22035160 Rhodospirillum rubrum Periplasmatic PHA depolymerases [7]
130002 Pseudomonas oleovorans Intracellular nPHAMCL depolymerases [18]
21689574 Pseudomonas putida   [17]
130019 Ralstonia pickettii Extracellular dPHASCL depolymerises (catalytic domain type 1) [29]
1777951 Alcaligenes faecalis   [23]
116744367 Bacillus megaterium   [48]
1730532 Paucimonas lemoignei   [20]
7385117 Paucimonas lemoignei   [31]
1657610 Paucimonas lemoignei   [15]
1621355 Paucimonas lemoignei   [15]
531464 Paucimonas lemoignei   [45]
531466 Paucimonas lemoignei   [45]
75538924 Pseudomonas stutzeri   [26]
5360565 Ralstonia pickettii   
1381030 Ralstonia pickettii   
4033618 Acidovorax sp. TP4 Extracellular dPHASCL depolymerises (catalytic domain type 2) [25]
7209864 Caldimonas manganoxidans   [33]
565666 Comamonas sp.   [19]
75340123 Delftia acidovorans   [21]
47078657 Schlegelella sp. KB1a   [27]
1389770 Streptomyces exfoliatus   [24]
88192747 Penicillium funiculosum   [16]
15788987 Paucimonas lemoignei Extracellular nPHASCL depolymerases [6]
34452163 Pseudomonas alcaligenes Extracellular dPHAMCL depolymerases [22]
29470160 Pseudomonas alcaligenes   [22]
21542177 Pseudomonas fluorescens   [30]