Figure 8
From: TCP: a tool for designing chimera proteins based on the tertiary structure information
The use of chimera proteins divided into two portions for epitope analysis. A: Projection images of a protein from two viewpoints. The lower is the image of that the upper is rotated around the horizontal axis by π/2. Three epitopes are postulated in the protein and indicated by a filled circle, a filled triangle and a filled box. Three CSs are indicated with a solid line, a broken line and a dotted line. B: The six divided portions with the three CSs are labelled with numbers. C: The expected binding pattern of each epitope indicated in A. A portion with the number shown in B is the original sequence and another is modified. For example, the area 1 means that the portion 1 is the original sequence and the portion 2 is the substituted sequence. It is expected that the antibodies which recognize one of the two epitopes labelled as the filled circle or the filled triangle bind the protein labelled as 1, but antibodies which recognize the epitope labelled as the filled box does not.