Figure 3
Distribution of spots as a function of the relative difference between two scans. The number of spots having a difference of intensity from one scan to the next in a series of eight successive scans (see P f, s, n in materials and methods) is plotted as a function of that difference, for slide #3. Each series of bars in the histogram increases by a relative difference of 1.25 percent of the adjusted intensity. Bars in light grey represent the results for images with one acquisition; bars in grey represent those for images obtained with two acquisitions of the same line and those in black for images obtained with three acquisitions. The broken line corresponds to a difference of ten percent from scan to scan. Part A is for Cy3 images (532 nm); part B for Cy5 images (635 nm).