Figure 5

The standard deviation of each pixel of "non-outlier" spots and of "outlier" spots. Spots derived from 8 successive scans with one, two or three acquisitions per line are shown from the left to the right. The height of each vertical bar corresponds to the standard deviation of one pixel within the scan series, graduations are indicated by white broken lines. Parts A1 and A2 are for "non outlier" spots of intensity F635 _adj equal to 1100 and F532 _adj equal to 1050, respectively; B1 and B2 are for "outlier" spots of intensities 1100 and 1050, respectively. For "outlier" spot B1 the maximum of the standard deviation decreases from 300 to 200 to 160 while in A1 it is lower and decreases from 280 to 160 to 100 going from one to three successive acquisitions. For "outlier" spot B2, the maximum of the standard deviation decreases from 240 to 200 to 160 while in A2 it is lower and decreases from 200 to 120 to 100 going from one to three successive acquisitions.