| | 5 microns resolution | 10 microns resolution |
|---|
PMT voltages | F635 | F532 | F635 | F532 |
400-400 | 0.0 | 0.0 | 0.0 | 0.0 |
500-500 | 0.0 | 0.0 | 0.7 | 1.4 |
600-600 | 1.4 | 0.5 | 6.5 | 3.2 |
700-700 | 3.7 | 1.8 | 11.5 | 4.0 |
- Results for slide #1. 400-400 corresponds to settings 400 V for the red dye (F635) and 400 V for the green dye (F532), respectively. The percentage of "outlier" spots, P
out
, is equal to: P
out
= N
out
/N with N
out
being the number of events where a spot is found to be an "outlier" between two successive scans and N = (Nscans -1) × n
spots
(Nscans is the number of scans in the series and n
spots
is the number of spots on the slide).
