Flowchart of our web-based and statistical strategy used to elucidate the relation between PKA encoding genes transcriptional profiles and oncogenic mutations. (A) Flow chart of our web-based and statistical strategy with indication of some of the databases (Source) used, the type of data analyzed (Input), the specific program and statistical test (Tool) used and the result obtained (Output). (B) Graphical representation of the block diagram summarizing functional interconnections within the PKA pathway module with indication of the expression level (geometric mean) of each gene belonging to the network -Strong (red), Average (black) and Low (green)- as identified by our analysis both in normal (B, left) and transformed samples (B, right). (C) Boxplots of the expression of PKA pathway-encoding genes in normal (C, left) and transformed (C, right) samples, grouped for functional classes (ADCY: adenylyl cyclase; AKAP: A-kinase anchor protein; PDE: phosphodiesterase; PRKACR: PKA regulatory subunit; PRKAC: PKA catalytic subunit). The represented value is the median. (D) Schematic representation of the TFBSs (color-coded) identified in the promoters of PKA pathway-encoding genes of normal and transformed samples. Each cartoon represents the promoter structure resulting from the merge of the TFBS identified in ≥ 70% of the gene promoters of all normal samples and transformed samples.