Figure 6

Measurements of promoter activity and growth kinetics for GFP reporter strains with high-temporal resolution. Transcriptional activity of wild-type Salmonella harboring plasmids with a transcriptional fusion between a promoterless gfp gene and the Salmonella promoters including phoP (blue), pmrd(green), slyB (red), pagC (cyan), ugd(magenta), ugtL (yellow) and mgtA (orange). Each experiment was conducted independently at least twice, and shown after preprocessing. The activity of each promoter is proportional to the number of GFP molecules produced per unit time per cell [dG _i (t)/dt]/OD _i (t)], where G _i (t) is GFP fluorescence from wild-type Salmonella strain 14028s culture and conditions described in Methods, and OD _i (t) is the optical density. The activity signal was smoothed by a polynomial fit (sixth order).