ICEP run time chart. (A) Run time analysis of different microarray versions containing an increasing number of features. The plot shows the resulting increase in ICEP run time for different IronChip versions. (B) A set of virtual arrays of 1000 to 9000 features was analyzed. We used a general tab delimited format. Robust statistical analysis included analysis of background noise, ratio cut-off, evaluation of multiple repetitions, detailed feature extraction and grouping results. ICEP Run time increases linearly with the increase of the total number of analyzed features. On average, ICEP evaluates 208 features per second.