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Table 3 EST P-call definition

From: The IronChip evaluation package: a package of perl modules for robust analysis of custom microarrays

Rule Nr. EST P-call Conditions Description
1 P P ≥ 60% P If more than 60% of features representing one EST have a p-call "P" then assign an EST P-call "P"
2 M M ≥ 60% M If more than 60% of features representing one EST have a p-call "M" then assign an EST P-call "M"
3 A A ≥ 60% A If more than 60% of features representing one EST have a p-call "A" then assign an EST P-call "A"
4 M not 1, 2 and 3: M + P ≥ 60% M If criteria 1, 2 and 3 do not apply and more than 60% of features representing one EST have the p-calls "M" and "P" than assign an EST P-call "M"
5 A not 1, 2, 3 and 4: M + A ≥ 60% A If criteria 1, 2, 3 and 4 do not apply and more than 60% of features representing one EST have the p-calls "M" and "A" than assign a EST P-call "A"
  1. Definition of a P-call flag (at the EST level) is based on the P-calls of individual features and allows to distinguish UP-/DOWN-/NONE-regulated ESTs from each other