Table 4 Dye-swap reliability flag definition
From: The IronChip evaluation package: a package of perl modules for robust analysis of custom microarrays
Dye-swap flag | Conditions |
|---|---|
Absent | EST shows the p-call "A" in the Cy5 and Cy3 experiment |
Non reliable | The Cy5 and the Cy3 experiment show identical regulatory behavior (both UP, both DOWN) |
Non regulated | EST does not show any regulation in both experiments |
TRUE | EST shows "P" or "M" p-calls and is UP-regulated in the Cy5 and DOWN-regulated in the Cy3 experiment, or vice versa. |
TRUE | EST shows "P" p-call and is UP-regulated or DOWN-regulated in the, Cy5 while the Cy3 experiment shows a tendency towards the correct direction based on the ratio cut-off value, or vice versa |
TRUE | EST shows "P" or "M" p-call, but both experiments show NONE-regulated with one is UP-regulated or DOWN-regulated other is NONE-regulated, a tendency of regulation towards the correct direction based on a ratio cut-off |