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Table 1 Sample results of de novo sequencing and verification.

From: A high-throughput de novo sequencing approach for shotgun proteomics using high-resolution tandem mass spectrometry

Scan #

De novo Sequencing

Verification

 

Residual Mass

Tag Sequence*

Residual Mass

Score

Tag Validity

Sequest Identification*

1616

1001.540

APAJG

146.112

8.7

Correct

K.RVFNVLTGD APAIG K.V

1629

1069.550

VJCJQ PK

44.049

5.3

N/A

N/A

1630

897.447

Q EKEVAAVJ

128.105

14.8

Incorrect

K.K LVAAVEKE GAGFDLGAYR.D

1635

980.533

EJVQ

146.109

5.3

Incorrect

K.MIHFVPRDNIVQ K.A

1636

358.233

DGJMVJA

137.071

22.1

Correct

R.HALVMLGD ALR.H

1640

1219.610

TSSMG

589.296

7.6

Correct

R.NYAQLGMSST PFYQSHGVASK.S

1641

830.437

TJFGA

146.113

11.5

Correct

K.IFTTRPDTLFGA K.F

1643

888.524

AGDGAK

147.079

10.4

Correct

R.FKAGDGA IVNGIAFR.S

1644

128.096

JFDVJA

271.174

19.3

Correct

K.KLFDVLA PR.Y

  1. * Matched sections between the sequence tags and the Sequest identifications are underlined.
  2. * Letter J represents amino acids L or I.
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BMC Bioinformatics

ISSN: 1471-2105

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