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Table 1 Sample results of de novo sequencing and verification.

From: A high-throughput de novo sequencing approach for shotgun proteomics using high-resolution tandem mass spectrometry

Scan # De novo Sequencing Verification
  Residual Mass Tag Sequence* Residual Mass Score Tag Validity Sequest Identification*
1616 1001.540 APAJG 146.112 8.7 Correct K.RVFNVLTGD APAIG K.V
1629 1069.550 VJCJQ PK 44.049 5.3 N/A N/A
1630 897.447 Q EKEVAAVJ 128.105 14.8 Incorrect K.K LVAAVEKE GAGFDLGAYR.D
1635 980.533 EJVQ 146.109 5.3 Incorrect K.MIHFVPRDNIVQ K.A
1636 358.233 DGJMVJA 137.071 22.1 Correct R.HALVMLGD ALR.H
1640 1219.610 TSSMG 589.296 7.6 Correct R.NYAQLGMSST PFYQSHGVASK.S
1641 830.437 TJFGA 146.113 11.5 Correct K.IFTTRPDTLFGA K.F
1643 888.524 AGDGAK 147.079 10.4 Correct R.FKAGDGA IVNGIAFR.S
1644 128.096 JFDVJA 271.174 19.3 Correct K.KLFDVLA PR.Y
  1. * Matched sections between the sequence tags and the Sequest identifications are underlined.
  2. * Letter J represents amino acids L or I.