A Sample Region of Simulated Peak Profiles Before and After Alignment. Alignment is shown with either PCANS-aligned or template-aligned peak profiles. Short, individually colored bars indicate a profile's peaks. Peak profiles were simulated from two groups having group-specific peaks, with 32 profiles in each group. The colorbar on the right indicates relative height (intensity) of the simulated peak profiles. The horizontal orange rectangle in panel A indicates the best overall individual peak profile that was used as the template for this alignment. Regions indicated by vertical cyan rectangles depict cases where Group 1 has unique peaks that differentiate it from Group 2.