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Table 1 Multi-way ANOVA table assessing components used in OTU methodologies.

From: Alignment and clustering of phylogenetic markers - implications for microbial diversity studies

Parameter Sum of Squares Degrees of freedom Mean Sq. F Prob >F
Distance threshold 0.4411 11 0.0401 23.0160 < 0.0001
MSA 0.0480 2 0.0240 13.7843 < 0.0001
Clustering 0.0099 2 0.0050 2.8503 0.0604
Distance correction < 0.0001 2 < 0.0001 0.0020 0.9980
Error 0.3171 182 0.0017   
Total 0.7910 199 0.0708   
  1. The factor with the largest effect on the quality of the OTUs was the distance threshold, followed by the MSA, and then the clustering algorithm. The distance correction explained < 0.01% of the variance and no statistically significant difference could be detected between the corrections (P = 0.998).