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Figure 9 | BMC Bioinformatics

Figure 9

From: Optimization algorithms for functional deimmunization of therapeutic proteins

Figure 9

Full-length Epo variant profile. Optimized 10-substitution Epo variant with ProPred epitope scoring and BLOSUM-based substitutions. x-axis: starting position of each 9-mer; y-axis: predicted number of alleles recognizing the 9-mer. Thin black bars indicate wild-type scores and thick orange bars indicate variant scores. Note: wild-type epitope scores are always greater than or equal to corresponding variant ones; i.e., we never introduce new epitopes. Blue ellipses indicate mutated positions (refer to Table 3). The line plot, from Tangri et al. [22] displays wild-type Epo antigenicity using ELISPOT assays, with black squares giving the number of alleles bound to overlapping 15-mers.

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