Table 1 Comparison between Detection Call and On/Off method using spiked-in genes.
From: A robust method for estimating gene expression states using Affymetrix microarray probe level data
| Â | Detection Call | On/Off | ||||
|---|---|---|---|---|---|---|
True concentration (pM) | Present | Marginal | Absent | X ≥ 11 | X ≤ 10 | Total |
0 | 1 | 0 | 58 | 4 | 55 | 59 |
0.25 | 10 | 0 | 49 | 27 | 32 | 59 |
0.5 | 21 | 4 | 33 | 44 | 14 | 58 |
1.0 | 22 | 1 | 36 | 48 | 11 | 59 |
2 | 37 | 3 | 19 | 52 | 7 | 59 |
4 | 48 | 2 | 9 | 56 | 3 | 59 |
8 | 58 | 1 | 0 | 59 | 0 | 59 |
16 | 59 | 0 | 0 | 59 | 0 | 59 |
32 | 59 | 0 | 0 | 59 | 0 | 59 |
64 | 50 | 0 | 0 | 50 | 0 | 50 |
128 | 50 | 0 | 0 | 50 | 0 | 50 |
256 | 59 | 0 | 0 | 59 | 0 | 59 |
512 | 68 | 0 | 0 | 68 | 0 | 68 |
1024 | 69 | 0 | 0 | 69 | 0 | 69 |
Total | 611 | 11 | 204 | 704 | 122 | 826 |