Figure 4
From: Shared probe design and existing microarray reanalysis using PICKY
A comparison of linear and nonlinear salt effects. The target and closest nontarget melting temperature differences of 50-mer probes calculated using (a) the linear salt effect equation and (b) the nonlinear salt effect equation are expressed as a function of targeting sequence locations and salt concentrations. For example, the 50-mer probe targeting location 650-699 under 0 salt concentrations has a calculated melting temperature difference of either 25°C or 21°C using the two different equations. The temperature difference exhibits no dependence on salt concentration when calculated using the linear salt effect equation but becomes sensitive to salt concentration when calculated using the nonlinear salt effect equation.