Figure 5

An example of sequences sharing the same probe. In this probe target region view on Gene 685, Genes 657, 1113 and 2212 are collected at the top and shown to contain the same target region. The shorter DNA fragments below them are detected nontargets to the probe. When a fragment is moused over, PICKY dynamically displays an alignment of the fragment-containing sequence (e.g., Gene 1113 as shown) with the target sequence (i.e., Gene 685). The melting temperatures of the probe with all its targets and nontargets are shown in the TEMP column and are used to sort the list.