Figure 3
From: Comparative performances of DNA barcoding across insect orders
Relationships between barcode length and identification success. Non-linear regression of the identification success obtained by considering the full DNA barcode (550-658 bp) and three non-overlapping "mini-barcodes" (MB1: 220 bp, MB2: 219 bp, MB3: 219 bp). Each mini-barcode was reduced at both 5' and 3' ends in order to obtain fragments of 164 bp, 110 bp, 55 bp and 22 bp (corresponding to approximately to 75%, 50%, 25% and 10% of the initial mini-barcode length). For each fragment the proportion of correct identifications was calculated according to three identification criteria (BM: Best Match, BCM: Best Close Match, NJT: Neighbor-Joining Tree) and averaged across six insect orders (Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera, Orthoptera, SD as error bars). Regression curves were fitted following a first order exponential decay model y = y0 + ae(-x/t), where y0 = Y offset, a = amplitude, t = exponential time constant (see Table 3).