Figure 2

Comparison of the quantification performance of the WaveletQuant and the ASAPRatio for yeast extracts mixed in 1:1 ratio. A: Comparison of the quantification performance of yeast extracts mixed in 1:1 ratio between the WaveletQuant (right) and the ASAPRatio (left). The MS spectra OR20070625_HS_L-H-1-1_12.03299.03299.3 (+3 charge state) are illustrated. LC-MS chromatograms of the isotopically light and heavy peptide partners are shown. Raw chromatograms are plotted in red, smoothed chromatograms in blue, areas used for calculating abundance ratio of the charge state in green, and backgrounds in cyan. On the top is peptide abundance ratio. On the right are start and end scan numbers, background, elution time of the isotopically light and heavy peptide partners, acceptance, abundance ratio, and weight of the charge states. Users may change scan numbers, background levels, and acceptance of the charge state. B: Comparison of the quantification performance of yeast extracts mixed in 1:1 ratio between the WaveletQuant (right) and the ASAPRatio (left). MS spectra OR20070625_HS_L-H-1-1_12.10969.10969.2 (+2 charge state) are illustrated.