From: Selection of optimal reference genes for normalization in quantitative RT-PCR
 |  |  |  |  |  |  |  |  |  |  |  |  | Min Var of NF1 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Scenario | Std Dev | Correlation Matrix of R | Total Covariance Matrix V | No Genes | True | Uncorr 2 | ||||||||
 | 0.30 | 1 | 0 | 0 | 0 | 0 | 0.25 | 0.16 | 0.16 | 0.16 | 0.16 | 1 | 0.250 | 0.250 |
Uncorrelated R | 0.35 | 0 | 1 | 0 | 0 | 0 | 0.16 | 0.28 | 0.16 | 0.16 | 0.16 | 2 | 0.213 | 0.133 |
Sample Random | 0.80 | 0 | 0 | 1 | 0 | 0 | 0.16 | 0.16 | 0.80 | 0.16 | 0.16 | 3 | 0.255 | 0.148 |
Effect Var = 0.16 | 0.90 | 0 | 0 | 0 | 1 | 0 | 0.16 | 0.16 | 0.16 | 0.97 | 0.16 | 4 | 0.264 | 0.144 |
 | 1.00 | 0 | 0 | 0 | 0 | 1 | 0.16 | 0.16 | 0.16 | 0.16 | 1.16 | 5 | 0.267 | 0.139 |
 | 0.60 | 1 | 0.2 | 0.2 | 0.2 | 0.2 | 0.38 | 0.10 | 0.11 | 0.15 | 0.16 | 1 | 0.380 | 0.380 |
Corr Coef = 0.2 | 0.70 | 0.2 | 1 | 0.2 | 0.2 | 0.2 | 0.10 | 0.51 | 0.13 | 0.17 | 0.19 | 2 | 0.275 | 0.223 |
Sample Random | 0.75 | 0.2 | 0.2 | 1 | 0.2 | 0.2 | 0.11 | 0.13 | 0.58 | 0.19 | 0.20 | 3 | 0.239 | 0.164 |
Effect Var = 0.02 | 1.10 | 0.2 | 0.2 | 0.2 | 1 | 0.2 | 0.15 | 0.17 | 0.19 | 1.23 | 0.28 | 4 | 0.275 | 0.169 |
 | 1.20 | 0.2 | 0.2 | 0.2 | 0.2 | 1 | 0.16 | 0.19 | 0.20 | 0.28 | 1.46 | 5 | 0.301 | 0.167 |
 | 0.42 | 1 | -0.2 | -0.2 | 0.2 | 0.2 | 0.28 | 0.06 | 0.06 | 0.15 | 0.15 | 1 | 0.276 | 0.276 |
Corr Coef = ±0.2 | 0.45 | -0.2 | 1 | -0.2 | 0.2 | 0.2 | 0.06 | 0.30 | 0.06 | 0.15 | 0.15 | 2 | 0.176 | 0.145 |
Sample Random | 0.48 | -0.2 | -0.2 | 1 | 0.2 | 0.2 | 0.06 | 0.06 | 0.33 | 0.16 | 0.16 | 3 | 0.141 | 0.101 |
Effect Var = 0.1 | 0.60 | 0.2 | 0.2 | 0.2 | 1 | 0.2 | 0.15 | 0.15 | 0.16 | 0.46 | 0.17 | 4 | 0.166 | 0.086 |
 | 0.60 | 0.2 | 0.2 | 0.2 | 0.2 | 1 | 0.15 | 0.15 | 0.16 | 0.17 | 0.46 | 5 | 0.175 | 0.073 |
 | 0.30 | 1 | -0.4 | 0.0 | 0.0 | 0.0 | 0.25 | 0.11 | 0.16 | 0.16 | 0.16 | 1 | 0.250 | 0.090 |
Corr Coef = ±0.4 | 0.40 | -0.4 | 1 | 0.0 | 0.0 | 0.0 | 0.11 | 0.32 | 0.16 | 0.16 | 0.16 | 2 | 0.199 | 0.063 |
Sample Random | 0.60 | 0.0 | 0.0 | 1 | 0.0 | 0.0 | 0.16 | 0.16 | 0.52 | 0.16 | 0.16 | 3 | 0.217 | 0.068 |
Effect Var = 0.16 | 0.70 | 0.0 | 0.0 | 0.0 | 1 | 0.4 | 0.16 | 0.16 | 0.16 | 0.65 | 0.38 | 4 | 0.223 | 0.069 |
 | 0.80 | 0.0 | 0.0 | 0.0 | 0.4 | 1 | 0.16 | 0.16 | 0.16 | 0.38 | 0.80 | 5 | 0.244 | 0.070 |
 | 0.40 | 1 | 0.4 | 0.4 | 0.4 | 0.4 | 0.26 | 0.18 | 0.21 | 0.23 | 0.24 | 1 | 0.260 | 0.260 |
Corr Coef = 0.4 | 0.50 | 0.4 | 1 | 0.4 | 0.4 | 0.4 | 0.18 | 0.35 | 0.24 | 0.26 | 0.28 | 2 | 0.243 | 0.153 |
Sample Random | 0.70 | 0.4 | 0.4 | 1 | 0.4 | 0.4 | 0.21 | 0.24 | 0.59 | 0.32 | 0.35 | 3 | 0.274 | 0.133 |
Effect Var = 0.1 | 0.80 | 0.4 | 0.4 | 0.4 | 1 | 0.4 | 0.23 | 0.26 | 0.32 | 0.74 | 0.39 | 4 | 0.302 | 0.121 |
 | 0.90 | 0.4 | 0.4 | 0.4 | 0.4 | 1 | 0.24 | 0.28 | 0.35 | 0.39 | 0.91 | 5 | 0.331 | 0.114 |