Figure 5

A comparison of different background correction methods. ROC plots for data processed with and without local background correction for the series A 33:67 mixture (top left) and series B 64:36 mixture (top right) are shown. These curves were calculated using the relevant true positive and true negative sets outlined in Figure 3 and Methods. In both cases, locally background corrected data (black line) offers more true positives than data which has not been background corrected (gray line). Looking at the results from all mixtures, we see that for series A (bottom left) and series B (bottom right), local background correction gives more true positives in almost all situations when performance is measured using area under the ROC curve.