Figure 7
Hook representation of the washing parameters: The figure shows the PM/MM-difference plot of the smoothed intensities before washing (Δ(t = 0) = logIPM/IMM), of the asymptotic washing level (Δw = log(w∞PM/w∞MM)) and of the decay times (Δτ = -(τ PM - τ MM)) as a function of the mean intensity, Σ. These hook plots reveal the typical hybridization regimes: non-specific (N), mixed (mix), specific (S), saturation (sat) and asymptotic (as) as indicated in the figure. The indicated 'percent values' estimate the degree of decrease in terms of the final level compared with the maximum. The dashed curves are theoretical fits using Eq. (20) (Δ(t = 0)) and Eqs. (37)-(38) (Δw), respectively. Note that theory predicts an asymmetric shape of Δw compared with the symmetric shape of Δ(t = 0) in agreement with the experimental curves.