The experimental validation of the IKK Kinase Assay. The experimental validation of the IKK Kinase Assay under three different doses of LPS at the time points of 0, 0.5, 1, 2, 4, 6, and 12 hours. The blue lines are obtained by Image Q (GE Healthcare) that represents the western blot results of IKK activity. The green dotted lines represent the simulation results from forward engineering modeling framework (ODE model). The patterns of IKK activity from both the IKK Kinase Assay and the computational results are consistence. The ten-fold and hundred-fold dose of LPS appears not to trigger an extremely intense cellular response but induce periods of slow decay and non-decreasing inflammatory response between 4 to 6 hours respectively.