Application of the boundary delimitation algorithm to live cells. A: Top views of the original data of four different OPCs obtained by pulse-mode SICM in floating backstep configuration. Orange lines indicate y (x, θh), blue dots indicate C90. B: Top views of the rotated, translated and interpolated data from the corresponding scans from A. The height of each scan and its corresponding rotated data is indicated by the color bars in A. C: Approximated soma area of the corresponding scans. The contribution of each pixel to the entire soma volume is indicated by the color bars at the bottom of each panel.