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Figure 9 | BMC Bioinformatics

Figure 9

From: A boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning probe microscopy

Figure 9

Application of boundary delimitation algorithm to simulated objects. A-C: Half-ellipsoids with the corresponding radii r x = ry = r0 (A), r x = 1.25 r0; ry = 0.8 r0 (B) and r x = 0.8 r0; r y = 1.25 r0 (C). The radius in z-direction is r z = r0. D-F: Hemisphere/half-ellipsoids from A-C with additional extensions. G: Normalized volume (Vn) computed by the boundary delimitation algorithm (BDA) as well as by thresholding simulating a half-ellipsoid with the radii r x = t r0 and r z = 1/t r0 for 1 ≤ t ≤ 2 and Δt = 0.05. Corresponding thresholds were 0.4 r z and 0.4 r0, respectively. H: Volumes of the objects from D-F computed by the BDA (blue) and by thresholding (red) using a threshold of 0.4 rz. Gray boxes indicate erroneously determined changes in volume when the shape of the object changes as indicated by the respective arrows. I: The addition of the extensions changes the volume of the simulated cell soma with respect to the mere half ellipsoid as indicated by the red area.

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